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基本信息
| 产品名称 | DNA酶I 来源于牛胰腺(过滤) |
|---|---|
| 英文名称 | Deoxyribonuclease I from Bovine Pancreas(Filtered) |
| 别名 | 脱氧核糖核酸酶 I |
| 英文别名 | Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I |
| 规格或纯度 | ≥2,000 Kunitz units/mg dry weight |
| 运输条件 | 冰袋运输 |
| 单位定义 | 1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthingto |
| 酶学委员会编号 | 3.1.21.1 |
| 生化机理 | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity. |
一般描述
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
安全信息
| 个人防护设备 | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| WGK | 3 |
相关属性
| CAS编号 | 9003-98-9 |
|---|---|
| 敏感性 | 易潮湿 |
| 储存温度 | 2-8°C储存 |
| RTECS | RF0750000 |
| MDL号 | MFCD00130918 |
| 分子式 | |
| EC号 | 232-667-0 |
| 品牌 | 阿拉丁 |
| PubChem CID | 597667 |