45.00
5,5'二硫代双(2-硝基苯甲酸)(DTNB) 98%
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价格
45.00
货号
D105559-1g
市场价
0.00
品牌
阿拉丁
销量
0
库存量
100
CAS号
69-78-3
分子式
C14H8N2O8S2
分子量
396.35

基本信息

产品名称5,5'二硫代双(2-硝基苯甲酸)(DTNB)
英文名称5,5'-Dithio bis-(2-nitrobenzoic acid)
别名双(3-羧基-4-硝基苯基)二硫;二硫代硝基苯甲酸;6,6′-二硝基-3,3′-二硫代苯甲酸
英文别名3-Carboxy-4-nitrophenyl disulfide; 6,6'-Dinitro-3,3'-dithiodibenzoic acid; Bis(3-carboxy-4-nitrophenyl) disulfide;DTNB; Ellman’s Reagent; DTNB Ellman’s Reagent
应用巯基测定,是测定蛋白质、多肽和组织中巯基的灵敏试剂。也用于有机磷农药中毒的监测(胆碱酯酶测定)。
规格或纯度98%
运输条件冰袋运输

一般描述

产品应用:

Ellman′s试剂可用于乙酰胆碱酯酶的检测,并可对半胱氨酸残基进行特异性标记。该试剂是测定蛋白质、多肽和组织中游离巯基含量的敏感试剂。


Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights: ?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product ?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm) ?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

安全信息

标识符号 GHS07
警示性声明P261,P305+P351+P338
危害声明H315,H319,H335
个人防护设备dust mask type N95 (US),Eyeshields,Gloves
WGK3
Merck Index3551

相关属性

CAS编号69-78-3
敏感性热敏感
熔点240-245°C
溶解性Soluble in 100% ethanol, dioxane, and Phosphate buffer.
储存温度2-8°C储存,干燥
Reaxys-RN1896821
RTECSDG9650000
MDL号MFCD00007140
密度1.6412
分子量396.35
分子式C14H8N2O8S2
EC号200-714-4
品牌阿拉丁
PubChem CID6254


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