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英文名称：Ponceau S；C.I. 27195；3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid tetrasodium salt
其他名称：猩红S；酸性红112；3-羟基-4-[2-磺酸基-4-(4-磺酸苯基偶氮)苯基偶氮]-2,7-萘二磺酸四钠盐
CAS号：6226-79-5
C22H12N4Na4O13S4=760.57
级别：High purity grade
Em(520nm，H2O)：＞27000
干燥失重：≤5.0%
生物染色试验：合格
水溶解试验Solubility (0.1%, Water)：合格
性状：波尔多红至暗红或棕色粉末，溶于水和乙醇，水溶液呈玫红色。最大吸收波长520(352) nm。有刺激性
用途：生化研究。生物染色，乙酸纤维素上电泳后的蛋白质带快速染色。丽春红S用于对硝酸纤维素或PVDF膜（Western blots)上的蛋白质进行染色。

保存：RT

Ponceau S

CAS Number: 6226-79-5

Storage Temperature: Room Temperature

Product Description :

Appearance: Deep red crystalline powder

Molecular formula: C22H12N4Na4O13S4

Molecular weight: 760.57

Ponceau S is a red, water-soluble, anionic diazo dye that is made by coupling diazotized 4-amino-1,1’-azobenzene-3,4’-disulfonic acid (Acid Yellow 9) with 2-naphthol-3,6-disulfonic acid(R acid)and converting the coupled product to the tetrasodium salt.

Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and is widely used in gel electrophoresis. It also binds non-covalently to non-polar regions in the protein.

Ponceau S may not be as sensitive of a protein stain as Coomassie Blue. It is superior to Acid Fuchsin in the VanGieson stain.

Preparation Instructions :

It is soluble in water (1 mg/ml), yielding a clear solution.

Procedure:

A solution as low as 1% ponceau S can be made in 7% acetic acid (w/w) for staining polyacrylamide gels. The gel can be left in this solution for eight hours. However, prior to placing the gel in this staining mixture, the proteins on the gels should be fixed with a fixative agent such as methanol:acetic acid or TCA.

Destaining of the gel can occur in 7% (v/v) acetic acid:water mixture with many changes of the destaining solution.

Storage/Stability:

Recommended storage is at room temperature. The shelf-life should be at least two years.

Precautions and Disclaimer :

For Laboratory Use Only. Not for drug, household or other uses.