名称 2.5%戊二醛固定液,电镜专用(Glutaraldehyde Fixed Solution)
保存 2-8℃避光保存,有效期12个月
产品简介
戊二醛固定液会引起蛋白质α-螺旋结构变形,不利于过氧化物酶染色,速度快,渗透力差。Phygene 2.5%戊二醛固定液,电镜专用(Glutaraldehyde Fixed Solution)主要由戊二醛、磷酸盐等组成,pH7.2~7.4,该固定液对细胞核、细胞浆的细微结构固定效果好,是最常用的标准戊二醛固定液,经常用于电镜标本的固定。
1、 根据实验具体要求操作。
3、 稍大标本应适当延长固定时间。
1、 戊二醛固定液有一定腐蚀性,请在通风较好的环境下小心操作, 避免吸入。
3、 取出新鲜组织后,应及时固定。无法及时固定时,应保存于生理盐水中及时送检。
<p background-color:#ffffff;"="" style="margin-bottom: 10px; color: rgb(43, 43, 43);">Glutaraldehyde is used in biochemistry applications as an amine-reactive homobifunctional crosslinker and fixative prior to SDS-PAGE, staining, or electron microscopy. It kills cells quickly by crosslinking their proteins and is usually employed alone or mixed with formaldehyde as the first of two fixative processes to stabilize specimens such as bacteria, plant material, and human cells. A second fixative procedure uses osmium tetroxide to crosslink and stabilize cell and organelle membrane lipids. Fixation is usually followed by dehydration of the tissue in ethanol or acetone, followed by embedding in an epoxy resin or acrylic resin.