苏科商城

草酸铵结晶紫染色液是革兰氏染色法中的一种成分。革兰阳性菌染色中，细胞经草酸铵结晶紫染色后再经Gram碘液处理，形成不溶性复合物，该复合物不能通过细胞壁，不易被脱色，所以保持紫色。染色后细菌与环境形成鲜明对比，可以清楚地观察到细菌的形态、排列及某些结构特征，而用以分类鉴定。

操作步骤(仅供参考)

染色结果：革兰氏阳性菌：蓝色至紫色；革兰氏阴性菌：红色。

注意事项

结晶紫染色液配制：结晶紫乙醇饱和液(结晶紫2g溶于20mL95%乙醇中)20mL，1%草酸铵水溶液80mL 将两液混匀置24h后过滤即成。

Crystal violet or gentian violet (also known as methyl violet 10B or hexamethyl pararosaniline chloride) is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic properties and was formerly important as a topical antiseptic. The medical use of the dye has been largely superseded by more modern drugs, although it is still listed by the World Health Organization.

When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590 nm and an extinction coefficient of 87,000 M−1 cm−1. The colour of the dye depends on the acidity of the solution. At a pH of 1.0, the dye is green with absorption maxima at 420 nm and 620 nm, while in a strongly acidic solution (pH of −1), the dye is yellow with an absorption maximum at 420 nm.