苏科商城

名称 冰冻切片快速抗原修复液 (1×) / Quick Antigen Retrieval Solution for Frozen Sections

存储 常温运输与保存

产品简介

从原理上来看，无论冰冻切片还是细胞爬片等，只要是用多聚甲醛、甲醛或其它醛类试剂固定的样品，进行抗原修复都会有效去除蛋白之间的交联，充分暴露抗原表位，从而改善免疫染色效果。

本产品特别适合用于冰冻切片的抗原修复并用于后续的免疫染色。

使用说明：

2， 将切片浸泡在抗原修复液中，室温孵育 5min。

4， 封闭液封闭切片。

1、 切片浸泡在1×抗原修复液中，最佳的加热时间需根据不同的样品和目的蛋白自行摸索。

3、 为了您的安全和健康，请穿实验服并戴一次性手套操作。

This is a simple method for antigen retrieval on aldehyde-fixed cryostat tissue sections or cultured cells. In many case, a brief 5 minutes pretreatment with Quick Antigen Retrieval Solution for Frozen Sections produced a dramatic increase in staining intensity by immunohistochemistry and immunofluorescence.

1. Rinse sections three times for 5 min each in PBS.

3. Rinse sections three times for 5 min each in PBS. It is important to wash sections well, otherwise residual SDS will denature the antibodies subsequently applied to sections.

5. Incubate in the primary antibody and complete immunohistochemical staining steps as desired.

References:

<p background-color:#ffffff;"="" style="margin-bottom: 10px; color: rgb(43, 43, 43);"> Brown D, Lydon J, McLaughlin M, Stuart-Tilley A, Tyszkowski R, Alper S (1996)   Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS). Histochem Cell Biol. 105(4):261-7. PubMed Abstract