苏科商城

货号 PH1519

名称 封闭用脱脂奶粉 Skimmed Milk Powder

规格 25g | 50g | 100g | 250g | 500g

存储 室温保存，保质期 2 年。常温运输。

产品简介

脱脂奶粉可用于制备微生物培养基；配制ELISA、western等实验的封闭液。western blot经过转印以后，膜上有了蛋白，但是还有很多地方是没有蛋白覆盖的，通过脱脂奶粉的蛋白孵育后，这些空白的膜都被脱脂奶粉的蛋白覆盖了，就防止后续孵育的一抗和二抗与印迹膜的非特异结合。在科研实验中，脱脂奶粉也是常用的生化试剂之一。

Fat-free powdered milk is used as a saturating agent to block nonspecific binding sites on supports like blotting membranes (nitrocellulose, polyvinylidene fluoride (PVDF) or nylon), preventing binding of further detection reagents and subsequent background. It may be referred as Blotto. The major protein of milk, casein, is responsible for most of the binding site saturation effect.