苏科商城

名称 希夫试剂（Schiff Reagent）

存储 4℃避光保存

产品简介

主要成分：主要由碱性品红、偏重亚硫酸钠、双蒸水等组成，过滤并且无色处理。

1、常规固定，常采用10% 的福尔马林，常规脱水包埋。

3、自来水冲洗2～3min，再用蒸馏水浸洗2 次。

5、自来水冲洗1 次，再用蒸馏水浸洗2 次。

7、自来水冲洗10min。

9、酸性乙醇分化液分化。

11、逐级常规乙醇脱水。

13、中性树胶封固。

染色结果：

1、对照切片可以用唾液，取唾液片（过滤后用）处理30～60 min 后，与其他切片共同入过碘酸溶液。

3、如果对照片采用其自身样本，对照片不经过碘酸溶液这一步，直接入Schiff Reagent。

1、Schiff Reagent 应置于4℃密闭保存，使用时避免接触过多的阳光和空气。

3、理想的Schiff Reagent接近于无色，如果试剂颜色呈极轻微粉红色亦可考虑使用。如试剂颜色呈明显的红色，应弃用。

5、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Schiff's reagent is named after Hugo Schiff, a German chemist who lived from 1834 until 1915, and who first used this preparation to test for the presence of aldehydes when performing organic chemistry reactions. The process of adding the reagent to a chemical solution is referred to as Schiff's test. It is one of the earliest organic chemistry dye tests to have been widely used for detection of a certain functional group in chemical solutions.

<p background-color:#ffffff;"="" style="margin-bottom: 10px; color: rgb(43, 43, 43);">Schiff's reagent is also sometimes used to dye organic tissues. When used for this purpose, several drops of the reagent are added to a slide containing human or animal cells. Those which contain aldehyde groups react with the reagent to give off a pink color, which makes it easier to observe detail under a light microscope. In most procedures, Schiff's reagent is used along with one or more other stains that react with different functional groups to create slides in which different cells take on different colors. This makes it easier to differentiate between various cell and tissue types.